OCT 2017
MOLECULAR MARKER-BASED DETECTION OF PRIMARY BACTERIAL ENDOSYMBIONT ASSOCIATED WITH THE COCONUT SCALE INSECTS, Aspidiotus destructor Signoret AND Aspidiotus rigidus Reyne (HEMIPTERA: DIASPIDIDAE)
Jillian E. Timple, Romnick A. Latina & Barbara L. Caoili
BIOLOGY OF A PHILIPPINE POPULATION OF Toxorhynchites splendens (Wiedemann) (DIPTERA: CULICIDAE: TOXORHYNCHITINAE) UNDER LABORATORY CONDITIONS WITH Aedes aegypti (L.) (DIPTERA: CULICIDAE: CULICINAE) AS PREY
Justine Bennette H. Millado, Augusto C. Sumalde, Loinda R. Baldrias, Jessamyn R. Adorada, and Barbara L. Caoili
SPRINGTAIL (COLLEMBOLA) ABUNDANCE IN TrichodermaENHANCED AND CONVENTIONAL CABBAGE (Brassica oleracea L.) PLOTS IN SARIAYA, QUEZON, PHILIPPINES
Marnelli P. Sotto-Alviola, Ireneo L. Lit, Jr., Merdelyn T. Caasi-Lit and Virginia C. Cuevas
BIOCHEMICAL CHARACTERIZATION OF MIDGUT RECEPTOR OF ASIAN CORN BORER Ostriniafurnacalis Guenee (Lepidoptera: Pyralidae) AND MODIFICATION OF DOMAIN III 6-sheet 13 of Bacillus thuringiensis CrylAb o-endotoxin
Edwin P. Alcantara and Aileen N. Bayot
Abstract
| Brush border membrane vesicles (BBMVs) were prepared from 4’h instar larvae of Asian corn borer Ostrinia furnacalis Guenee. SDS-PAGE analysis showed major protein bands with molecular weight in the range of 80 kDa to 250 kDa. Ligand blot analysis showed that biotinylated Cry1Ab o endotoxin strongly binds to a -250 kDa receptor protein from 0. furnacalis BBMV. Another receptor protein of -110 kDa weakly binds to Cry1Ab o endotoxin. At least two isoforms of the -250 kDa receptor protein have a pH range of 5.5 to 6.5 as determined by 2D-electrophoresis. Post-translational modification of 0. furnaca/is BBMV proteins was also detected as revealed by the presence of two glycosylated proteins with molecular weights of>250 kDa and 70 kDa, respectively. ABSTRACT Alanine scanning mutations were introduced in domain 11113-sheet 13 of Cry1Ab o-endotoxin. Results of bioassays showed a 5 to 7 fold reduction in insecticidal activity in Serine556Alanine, Serine ss ,Alanine and Serine,..Alanine Serine557Alanine mutants of Cry1Ab protein. The decrease in insecticidal activity suggests that Serine556 and Serine 557 are both important residues in the toxicity of Cry1 Ab o-endotoxin to 0. furnacalis. |
MORPHOMETRICS OF FOUR GENITAL TRAITS IN SOME PHILIPPINE POPULATIONS OF THE ASIAN CORN BORER, Ostrinia furnacalis [Gueriee] (Lepidoptera: Crambidae].
Gelyn D. Sapin, Barbara L. Caoili, and Jessamyn D. Recuenco-Adorada
SOIL-INHABITING MITES FROM THE QUEZON NATIONAL PARK AND SOUTHERN SIERRA MADRE MOUNTAINS IN QUEZON PROVINCE, LUZON IS., PHILIPPINES
Leonila A. Corpuz-Raros and Ireneo L. Lit, Jr
MASS-REARING OF BROAD MITE, Polyphagotarsonemus latus (Banks)(Tarsonemidae, Acari), ON ALUGBATI, Basella rubra L. (Basellaceae), WITH NOTES ON ITS FEEDING DAMAGE, DEVELOPMENT AND POPULATION GROWTH
Marcela M. Navasero and Mario V. Navasero
MULTIPLE MECHANISMS OF PERMETHRIN RESISTANCE IN THE RED FLOUR BEETLE, Tribolium castaneum Herbst
Alexander Joel G. Gibe and Naoki Motoyama
ABSTRACTS OF PAPERS
October 2013